Remediation of polycyclic aromatic hydrocarbons polluted soil by biochar loaded humic acid activating persulfate: performance, process and mechanisms.

The remediation for polycyclic aromatic hydrocarbons contaminated soil with cost-effective method has received significant public concern, a composite material, therefore, been fabricated by loading humic acid into biochar in this study to activate persulfate for naphthalene, pyrene and benzo(a)pyrene remediation. Experimental results proved the hypothesis that biochar loaded humic acid combined both advantages of individual materials in polycyclic aromatic hydrocarbons adsorption and persulfate activation, achieved synergistic performance in naphthalene, pyrene and benzo(a)pyrene removal from aqueous solution with efficiency reached at 98.2%, 99.3% and 90.1%, respectively. In addition, degradation played a crucial role in polycyclic aromatic hydrocarbons remediation, converting polycyclic aromatic hydrocarbons into less toxic intermediates through radicals of ·SO4-, ·OH, ·O2-, and 1O2 generated from persulfate activation process. Despite pH fluctuation and interfering ions inhibited remediation efficiency in some extent, the excellent performances of composite material in two field soil samples (76.7% and 91.9%) highlighted its potential in large-scale remediation.

Kitchen compost-derived humic acid application promotes ryegrass growth and enhances the accumulation of Cd: An analysis of the soil microenvironment and rhizosphere functional microbes.

Phytoremediation is an environmentally friendly and safe approach for remediating environments contaminated with heavy metals. Humic acid (HA) has high biological activity and can effectively complex with heavy metals. However, whether HA affects available Cd storage and the Cd accumulation ability of plants by altering the soil microenvironment and the distribution of special functional microorganisms remains unclear. Here, we investigated the effects of applying kitchen compost-derived HA on the growth and Cd enrichment capacity of ryegrass (Lolium perenne L.). Additionally, the key role of HA in regulating the structure of rhizosphere soil bacterial communities was identified. HA promoted the growth of perennial ryegrass and biomass accumulation and enhanced the Cd enrichment capacity of ryegrass. The positive effect of HA on the soil microenvironment and rhizosphere bacterial community was the main factor promoting the growth of ryegrass, and this was confirmed by the significant positive correlation between the ryegrass growth index and the content of SOM, AP, AK, and AN, as well as the abundance of rhizosphere growth-promoting bacteria such as Pseudomonas, Steroidobacter, Phenylobacterium, and Caulobacter. HA passivated Cd and inhibited the translocation capacity of ryegrass. The auxiliary effect of resistant bacteria on plants drove the absorption of Cd by ryegrass. In addition, HA enhanced the remediation of Cd-contaminated soil by ryegrass under different Cd levels, which indicated that kitchen compost-derived HA could be widely used for the phytoremediation of Cd-contaminated soil. Generally, our findings will aid the development of improved approaches for the use of kitchen compost-derived HA for the remediation of Cd-contaminated soil.

Effect of soil organic matter-mediated electron transfer on heavy metal remediation: Current status and perspectives.

Soil contamination by heavy metals poses major risks to human health and the environment. Given the current status of heavy metal pollution, many remediation techniques have been tested at laboratory and contaminated sites. The effects of soil organic matter-mediated electron transfer on heavy metal remediation have not been adequately studied, and the key mechanisms underlying this process have not yet been elucidated. In this review, microbial extracellular electron transfer pathways, organic matter electron transfer for heavy metal reduction, and the factors affecting these processes were discussed to enhance our understanding of heavy metal pollution. It was found that microbial extracellular electrons delivered by electron shuttles have the longest distance among the three electron transfer pathways, and the application of exogenous electron shuttles lays the foundation for efficient and persistent remediation of heavy metals. The organic matter-mediated electron transfer process, wherein organic matter acts as an electron shuttle, promotes the conversion of high valence state metal ions, such as Cr(VI), Hg(II), and U(VI), into less toxic and morphologically stable forms, which inhibits their mobility and bioavailability. Soil type, organic matter structural and content, heavy metal concentrations, and environmental factors (e.g., pH, redox potential, oxygen conditions, and temperature) all influence organic matter-mediated electron transfer processes and bioremediation of heavy metals. Organic matter can more effectively mediate electron transfer for heavy metal remediation under anaerobic conditions, as well as when the heavy metal content is low and the redox potential is suitable under fluvo-aquic/paddy soil conditions. Organic matter with high aromaticity, quinone groups, and phenol groups has a stronger electron transfer ability. This review provides new insights into the control and management of soil contamination and heavy metal remediation technologies.

Insights into relationships between polycyclic aromatic hydrocarbon concentration, bacterial communities and organic matter composition in coal gangue site.

Coal mining usually brought polycyclic aromatic hydrocarbons (PAHs) contamination. Relationships between the concentration of PAHs, bacterial communities and soil environmental factors were important for bioremediation of PAHs in soil. Total 4 kinds of soil samples with different concentrations of PAHs were selected from 7 typical coal gangue（CG） sites in Huainan, Anhui Province. The relationships between microorganisms, dissolved organic matter (DOM) composition and PAHs concentration were systematically analyzed in this work. Total 11 kinds of PAHs were enriched in the soil surface layer. That was attributed to the strong binding of soil organic matter (SOM) to PAHs. PAHs contamination reduced the diversity of soil microbial. The abundance of PAHs-degrading genera such as Arthrobacter decreased with the increasing concentration of PAHs. Mycobacterium increased with the increasing concentration of PAHs in all samples. The microbial activities decreased with increasing concentration of PAHs. The increasing contents of LWM-PAHs and DOM were beneficial to improve the activities of soil microbial. The increasing DOM aromaticity was beneficial to improve the bioavailability of PAHs according to the correlation analysis between PAHs content and DOM structural parameters. The obtained results provide a basis for better understanding the contamination characteristics and microbial communities of coal gangue PAH-contaminated sites.

The relationships between heavy metals and bacterial communities in a coal gangue site.

Coal is the main source of energy for China's economic development, but coal gangue dumps are a major source of heavy metal pollution. Bacterial communities have a major effect on the bioremediation of heavy metals in coal gangue dumps. The effects of different concentrations of heavy metals on the composition of bacterial communities in coal gangue sites remain unclear. Soil bacterial communities from four gangue sites that vary in natural heavy metal concentrations were investigated using high-throughput sequencing in this study. Correlations among bacterial communities, heavy metal concentrations, physicochemical properties of the soil, and the composition of dissolved organic matter of soil in coal gangue dumps were also analyzed. Our results indicated that Actinobacteriota, Proteobacteria, Chloroflexi, Acidobacteriota, and Gemmatimonadota were the bacterial taxa most resistant to heavy metal stress at gangue sites. Heavy metal contamination may be the main cause of changes in bacterial communities. Heavy metal pollution can foster mutually beneficial symbioses between microbial species. Microbial-derived organic matter was the main source of soil organic matter in unvegetated mining areas, and this could affect the toxicity and transport of heavy metals in soil. Polar functional groups such as hydroxyl and ester groups (A226-400) play an important role in the reaction of cadmium (Cd) and lead (Pb), and organic matter with low molecular weight (SR) tends to bind more to mercury (Hg). In addition to heavy metals, the content of nitrogen (N), phosphorus (P), and total organic carbon (TOC) also affected the composition of the bacterial communities; TOC had the strongest effect, followed by N, SOM, and P. Our findings have implications for the microbial remediation of heavy metal-contaminated soils in coal gangue sites and sustainable development.

Differential responses of the properties of soil humic acid and fulvic acid to nitrogen addition in the North China Plain.

Humus (HS) is an important component of soil organic matter. Humic acid (HA) and fulvic acid (FA) are two of the most important components of HS, as they substantially affect biogeochemical processes and the migration and transformation of pollutants in soil. Long-term nitrogen (N) addition can lead to changes in soil physical and chemical properties, affect the structural characteristics of soil HS (HA and FA), cause changes in the adsorption and migration of pollutants, and ultimately result in the continuous deterioration of the soil ecological environment. However, few studies have examined the effects of N addition on the structural characteristics of soil HS, including the responses of soil HA and FA to N addition. Here, we conducted a long-term positioning experiment with different levels of N addition (CK: 0 kg N ha-1 yr-1, LN: 100 kg N ha-1 yr-1, and HN: 300 kg N ha-1 yr-1) in typical farmland soils of the North China Plain to study the response of soil HA and FA to N addition. N addition altered the physical and chemical properties of soil (e.g., pH, SOC, TN, and enzyme activity), which affected the responses of the chemical structure, quality indexes, and composition distribution of soil HA and FA to N addition. Differences in the response to N addition between HA and FA were observed. The structural characteristics of FA were stronger in response to HN compared with those of soil HA. As the level of N added increased, soil FA degradation increased, the composition distribution changed, the aromatization degree and molecular weight decreased, and the molecular structure became simpler. The properties of soil HA did not significantly respond to N addition. Given increases in the global N input (N addition and N deposition), our results have implications for agricultural fertilization, soil management, and other activities.

Nitrogen addition increases the ecological and human health risks of PAHs in different fractions of soil in sewage-irrigated area.

Nitrogen (N) is one of the most important nutrients required by soil and crops. N addition improves soil quality and fertility. However, long-term N addition changes the soil environment, which may affect the adsorption and accumulation of organic pollutants in soil. The adsorption of pollutants by the light fractions (LF) and heavy fractions (HF) of soil, and their resulting risks, might differ. In addition, several organic pollutants, especially PAHs, accumulate in farmland soil under long-term sewage irrigation. However, few studies have examined the response of PAHs to N addition in soil in sewage-irrigated areas, including whether there is a difference in the response of the LF and HF of soil. Here, a long-term experiment was carried out in farmland soils in typical sewage-irrigated areas to reveal the adsorption and accumulation of PAHs in bulk soil, LF, and HF, and the human health and ecological environment risks posed by PAHs under different levels of N addition. Under long-term N addition, the concentration of PAHs in soil increased and fluctuated from 7598 µg kg-1 to 10,414 µg kg-1. Significant differences in the PAHs concentration in the LF (5048 µg kg-1 to 1889 µg kg-1) and HF (2536 µg kg-1 to 8521 µg kg-1) and the human health and ecological risks of soil with N addition in typical sewage-irrigated areas were observed. The HF of soil was characterized by low carcinogenic and ecological risks. The results of our research provide insight into possible management actions that could be taken to enhance the environmental protection and safety of agricultural production activities, such as sustainability fertilization.